Neural stem cells NSCs reside in a unique microenvironment called the neurogenic niche and generate functional new neurons. The neurogenic niche contains several distinct types of cells and interacts with the NSCs in the subventricular zone SVZ of the lateral ventricle. From the isolated cells and microdissected choroid plexus, we obtained the secretory molecule expression profiling SMEP of each cell type using the Signal Sequence Trap method. We identified a total of genes encoding secretory or membrane proteins. Through the combination of multiple screening approaches, we identified a number of candidate genes with a potential relevance for regulating the NSC behaviors, which provide new insight into the nature of neurogenic niche signals. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
The molecular profiles of neural stem cell niche in the adult subventricular zone
The subventricular zone SVZ is the largest neurogenic niche in the adult mammalian brain. We found that the brain-enriched microRNA miR is an important regulator of the temporal progression of adult neurogenesis in mice. Knockdown of endogenous miR maintained purified SVZ stem cells as dividing precursors, whereas ectopic expression led to precocious and increased neuron formation. Furthermore, blocking miR function during regeneration led to hyperplasias, followed by a delayed burst of neurogenesis. We identified the SRY-box transcription factor Sox9 as being a physiological target of miR at the transition from the transit amplifying cell to the neuroblast stage. Sox9 overexpression abolished neuronal differentiation, whereas Sox9 knockdown led to increased neuron formation.
miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche
A neurogenic niche is a region where neurogenesis takes place. Neurogenesis refers to the entire set of events leading to the production of new neurons from precursor cell in the brain. The degree of neurogenesis depends on the interaction of the microenvironment niche with precursor cells that have neurogenic potential.